Journal: The Journal of biological chemistry

Article Title: Induction of ferritin synthesis in cells infected with Mengo virus.

doi: 10.1074/jbc.271.16.9851

Figure Lengend Snippet: FIG. 3. Mengo virus infection does not induce the transcrip- tion of ferritin genes. A, Northern blot analysis of 1 mg of cytoplasmic RNA isolated at various times after infection or mock-infection. The blot was sequentially hybridized with ferritin H cDNA, ferritin L cDNA, ribosomal protein L32 cDNA, and Mengo virus cDNA. The position of the ribosomal 18 and 26 S subunits are shown in the Mengo virus blot. B, graphical representation of the Northern blots in A showing mRNA levels in uninfected (M) and Mengo virus-infected (E) cells.

Article Snippet: Apoferritin L cDNA for probing Northern blots was synthesized using 1 mg of L cell mRNA, the reverse primer 59-CTTTCCAGGAAGTCACAGAG-39, which corresponds to base numbers 550–569 of the murine L apoferritin cDNA (42), and 200 units of Superscript reverse transcriptase (Life Technologies, Inc.).

Techniques: Virus, Infection, Northern Blot, Isolation

Journal: International Journal of Molecular Sciences

Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR

doi: 10.3390/ijms222111649

Figure Lengend Snippet: OP9 and NIH/3T3 cells show differential levels of OSMR and LIFR. ( A ) OP9 and NIH/3T3 cells were examined for LIFR and OSMR expression. BaF3 cells were used as a negative control. Protein molecular weight is labeled in kDa. Relative protein quantities are marked in red. ( B ) Microarray analysis of OP9 and NIH/3T3 cells showing Osmr and Lifr expression. The p -values were calculated by Transcriptome Analysis Console software. *** p < 0.001. ns = not significant. ( C ) OP9 and NIH/3T3 cells were analyzed for LIFR surface expression using FACS. ( D ) OP9 and NIH/3T3 cells were examined for expression of LIFR and OSMR in relation to different incubation periods with 10 ng/mL mOSM. Protein molecular weight is labeled in kDa. ( E ) OP9 and NIH/3T3 cells were examined for LIFR surface expression in relation to different incubation periods with 10 ng/mL mOSM. p -Values were calculated using one-way ANOVA and Bonferroni post-test. * p <0.05 and *** p < 0.001. ns = not significant.

Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).

Techniques: Expressing, Negative Control, Molecular Weight, Labeling, Microarray, Software, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR

doi: 10.3390/ijms222111649

Figure Lengend Snippet: OSMR downregulation attenuates OSM effects on proliferation. ( A , B ) Immunoblot quantifying the shRNA-induced knockdown of Osmr (n = 4) and Lifr (n = 5) in ( A ) OP9 and ( B ) NIH/3T3 cells. The shRNAs Osmr 5 and Lifr 1 were used in subsequent experiments. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( C ) Relative quantification of S phase of OP9 and NIH/3T3 cells carrying a knockdown for either Osmr , Lifr, or a Renilla control in presence or absence of 10 ng/mL mOSM. Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. *** p < 0.001. ns = not significant. ( D ) Immunoblot quantifying the LIFR overexpression in OP9 and NIH/3T3 cells. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( E ) Lifr overexpressing OP9 and NIH/3T3 cells were examined for LIFR surface expression compared to cells carrying the mock control. ( F ) Relative quantification of S phase of OP9 and NIH/3T3 cells transduced with Lifr or empty vector (MiG). Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).

Techniques: Western Blot, shRNA, Knockdown, Molecular Weight, Labeling, Quantitative Proteomics, Control, Cell Cycle Assay, Over Expression, Expressing, Transduction, Plasmid Preparation